Single-cell analysis of signal transduction in CD4 T cells stimulated by antigen in vivo
Year: 2001
Type: article
Abstract: Flow cytometry was used to study signaling events in individual CD4 T cells after antigen recognition in the body. Phosphorylation of c-jun and p38 mitogen-activated protein kinase was detected within minutes in all antigen-specific CD4 T cells in secondary lymphoid tissues after injection of peptide antigen into the bloodstream. The remarkable rapidity of this response correlated with the finding that most naive T cells are in constant contact with dendritic antigen-presenting cells. Contrary to predictions from in vitro experiments, antigen-induced c-jun and p38 mitogen-activated protein kinase phosphorylation did not depend on CD28 signals and was insensitive to inhibition by cyclosporin A. Our results highlight the efficiency of the in vivo immune response and underscore the need to verify which signaling pathways identified in vitro actually operate under physiological conditions.
Institution: University of Minnesota
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Awards: G1642810537, Costimulatory Signals in CD4+ T Cell Activation, G2659273361, G2685430039, G3309604385, Tracking CD4+ Memory T Cells in Vivo, Costimulatory Signals in CD4+ T Cell Activation, Regulation of IL-2 Gene Expression in T-cell Anergy, G598227692, G9000299220, Immunology Training Program, Mechanisms of peripheral induction of T-cell tolerance
OpenAlex ID: W2081292558