Work

Induction of Cytolysis of Cultured Lymphoma Cells by Adenosine 3′:5′-Cyclic Monophosphate and the Isolation of Resistant Variants

Year: 1973

Type: article

Abstract: Cultured mouse lymphosarcoma cells are killed on exposure to 0.1 mM N(6),O(2')-dibutyryl-adenosine 3':5'-cyclic monophosphate. A population of cells resistant to the killing effect of dibutyryl cyclic AMP at concentrations as high as 1 mM was selected. The growth characteristics of the resistant cells were similar to those of the sensitive parental line. However, the resistant cells contain less cytoplasmic cyclic AMP-binding proteins and decreased cyclic AMP-stimulated protein kinase activity. It is proposed that transition from sensitivity to resistance to dibutyryl cyclic AMP in lymphoma cells is connected with a modification of the cyclic AMP-binding protein, which appears to be the regulatory subunit of the cyclic AMP-activated protein kinase.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.70.1.76

Authors: Violet Daniel, Gerald Litwack, Gordon M. Tomkins

Institutions: University of California, San Francisco, Weizmann Institute of Science, Temple University

Language: English

Topic: Phosphodiesterase function and regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2081301723

FWCI: 8.364

Cites: 12

Cited by: 169

Related to: 10