Year: 2011
Type: article
Abstract: Currently, there is no standardized panel for immunophenotyping myeloid cells in mouse spleen using flow cytometry. Markers such as CD11b, CD11c, F4/80, Gr-1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid populations. Flow cytometry and fluorescence-activated cell sorting (FACS) analysis demonstrated that Ly6G/Ly6C markers are superior to Gr-1 for identifying splenic neutrophils, eosinophils, and subsets of monocytes/macrophages. Moreover, these experiments showed that F4/80 is not required for identifying these myeloid subsets and that many of the commercially available preparations of anti-F4/80 antibodies stain poorly for this antigen in spleen. Taken together, we have now developed an informative flow cytometry panel that can be combined with other cell markers to further delineate subpopulations of mouse splenic myeloid cells. This panel will be highly useful to investigators in the flow cytometry field, as there is a critical need to standardize the analysis of myeloid cell subsets.
Source: Cytometry Part A
DOI: 10.1002/cyto.a.22012
Authors: Shawn Rose, Alexander Misharin, Harris Perlman
Institution: Northwestern University
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
Awards: G1187687215, Cardiovascular Regenerative Medicine, Postdoctoral Rheumatology Training, G2068121275, Therapeutic Role for P21 in Suppressing IL-1 Beta Mediated Pathologies, A novel and improved mouse system for studying macrophage specific gene deletion, G474454551, G5562136253, Role of BH3-Domain Proteins in the Effector Phase of RA
OpenAlex ID: W2081374651