Work

A simple borohydride/GC method for measuring sparteine metabolites in man.

Year: 1986

Type: article

Abstract: A simple borohydride/GC method was developed for phenotyping sparteine oxidation in man. The major metabolites of sparteine found in human urine, 2- and 5-dehydrosparteine, were converted quantitatively back to sparteine by sodium borohydride reduction. The amount of sparteine metabolites can be estimated from the difference of sparteine concentrations between the borohydride-treated and untreated urine samples. The coefficient of variation of this assay was estimated from repeated analyses to be +/- 3% within a day (intra-assay) and +/- 8% between days (inter-assay).

Source: British Journal of Clinical Pharmacology

DOI: 10.1111/j.1365-2125.1986.tb02828.x

Authors: T Inaba, A Vinks, SV Otton

Institution: University of Toronto

Language: English

Topic: Pharmacogenetics and Drug Metabolism

Subfield: Pharmacology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Medical Research Council Canada