Work

Polyclonal Prame-Specific Cytotoxic T Lymphocytes Generated Using Protein-Spanning Pools of Overlapping Pentadecapeptides Target Chronic Myeloid Leukemia

Year: 2009

Type: article

Abstract: Following allogeneic hematopoietic stem cell transplantation, GVL is likely mediated not only by alloreactive T cells, but also by donor lymphocytes recognizing tumor associated antigens overexpressed by leukemic cells. Selective expansion of tumor-specific CTLs could augment GVL without increasing the risk of GVHD. The cancer testis antigen PRAME is a potential target for adoptive immunotherapy of hematologic and solid tumors. We have shown that PRAME-CTL can be expanded ex vivo, using a combination of artificial antigen presenting cells (aAPC) (K562 cell line genetically modified to express the HLA-A∗02 and costimulatory molecules) and cytokines.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2008.12.182