Work

Recognition of cysteine‐containing peptides through prompt fragmentation of the 4‐dimethylaminophenylazophenyl‐4′‐maleimide derivative during analysis by MALDI‐MS

Year: 2003

Type: article

Abstract: Under specified UV-MALDI conditions, the 4-dimethylaminophenylazophenyl-4'-maleimide (DABMI) derivative of cysteine-containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previously by others, derivatization with chromophoric DABMI allows cysteine-containing peptides and proteins to be tracked during HPLC by absorbance at upper UV and visible wavelengths. Here, we describe methodology by which these same peptide derivatives can be recognized by their distinctive MALDI mass spectral fragmentation pattern, then mass mapped, allowing for easy, rapid identification of cysteine-containing peptides.

Source: Protein Science

DOI: 10.1110/ps.0301403

Authors: Chad R. Borges, J. Throck Watson

Institution: Michigan State University

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G1724985691, DISULFIDE STRUCTURE OF BIOMEDICALLY IMPORTANT PROTEINS