Work

Alpha-bungarotoxin-horseradish peroxidase conjugate: preparation, properties and utilization for the histochemical detection of acetylcholine receptors.

Year: 1979

Type: article

Abstract: A method is presented for the efficient conjugation of horseradish peroxidase to alpha-bungarotoxin. The 1:1 molar conjugate obtained is purified to completion by gel filtration on Sephadex G-100, followed by ion exchange chromatography on CM-Sephadex. The conjugate retains half of the activity of unmodified horseradish peroxidase and binds effectively to the nicotinic acetylcholine receptor of muscle. The conjugate is proven to be useful reagent for the histochemical staining of the receptor on muscle fibers for light and electron microscopy.

Source: Journal of Histochemistry & Cytochemistry

DOI: 10.1177/27.4.376692

Authors: Z Vogel, M Towbin, M P Daniels

Institutions: National Institutes of Health, National Institutes of Health Clinical Center, Weizmann Institute of Science

Language: English

Topic: Nicotinic Acetylcholine Receptors Study

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2082025293

FWCI: 1.034

Cites:

Cited by: 17

Related to: 10