Carbohydrate requirement for expression and stability of acetylcholine receptor on the surface of embryonic muscle cells in culture.
Year: 1980
Type: article
Abstract: We have investigated the significance of protein glycosylation for metabolism of acetylcholine receptors (AcChoR) in primary cultures of embryonic chicken muscle cells. Tunicamycin, a specific inhibitor of the glycosylation of asparagine residues on glycoproteins, decreased AcChoR accumulation and accelerated its degradation. In contrast, there was no evidence that tunicamycin treatment affected AcChoR biosynthesis, intracellular transport, or incorporation into surface membranes. Leupeptin, an inhibitor of intracellular proteases, markedly increased accumulation of AcChoR on the external surface of muscle cells treated with tunicamycin. Our findings indicate that impairment of protein glycosylation prevents accumulation of AcChoR by increasing its susceptibility to degradation by cellular proteases.
Authors: J M Prives, K Olden
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Muscular Dystrophy Association
OpenAlex ID: W2082435269