Work

Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions

Year: 1996

Type: article

Abstract: Amphipols are a new class of surfactants that make it possible to handle membrane proteins in detergent-free aqueous solution as though they were soluble proteins. The strongly hydrophilic backbone of these polymers is grafted with hydrophobic chains, making them amphiphilic. Amphipols are able to stabilize in aqueous solution under their native state four well-characterized integral membrane proteins: (i) bacteriorhodopsin, (ii) a bacterial photosynthetic reaction center, (iii) cytochrome b6f, and (iv) matrix porin.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.93.26.15047

Authors: Christophe Tribet, Roland Audebert, Jean-Luc Popot

Institutions: Centre National de la Recherche Scientifique, Institut de Biologie Physico-Chimique, Université Pierre-et-Marie-Curie

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2082547742

FWCI: 1.273

Cites: 22

Cited by: 501

Related to: 10