Work

Concatenated logic gates using four coupled biocatalysts operating in series

Year: 2006

Type: article

Abstract: The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0608319103

Authors: Tamara Niazov, Ronan Baron, Eugenii Katz, Oleg Lioubashevski, Itamar Willner

Institution: Hebrew University of Jerusalem

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2082841173

FWCI: 6.522

Cites: 25

Cited by: 203

Related to: 10