Work

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Year: 2008

Type: article

Abstract: We demonstrate an inverse light-scattering analysis procedure based on using the T-matrix method as a light-scattering model. We measure light scattered by in vitro cell monolayers using angle-resolved low-coherence interferometry (a/LCI) and compare the data to predictions of the T-matrix theory. The comparison yields measurements of the equal volume diameter and aspect ratio of the spheroid cell nuclei with accuracy comparable to quantitative image analysis of fixed and stained samples. These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool.

Source: Optics Letters

DOI: 10.1364/ol.33.002452

Authors: Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, Adam Wax

Institutions: Duke University, Duke Medical Center

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Cancer Institute

Awards: G2882757739, CAREER: Low Coherence Light Scattering for Biophotonics, G9000123140