Work

The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase

Year: 2011

Type: article

Abstract: Phenoloxidase is a key enzyme of melanization catalyzing the oxidation of phenols. Phenylthiourea (PTU) is the well-known and widely used inhibitor of phenoloxidase. However, the mechanism of its action is not quite clear. In the present work, the effect of PTU on the enzymatic oxidation of 3-(3,4-dihydroxyphenyl)-l-alanine (DOPA) by phenoloxidase was studied by spectrophotometric methods. The inhibition constant of PTU was estimated as 0.21 ± 0.09 µM and the competitive type of inhibition was determined for this reaction.

Source: Journal of Enzyme Inhibition and Medicinal Chemistry

DOI: 10.3109/14756366.2011.576010

Authors: Alexandra D. Ryazanova, Alexander A. Alekseev, Irina A. Slepneva

Institution: Institute of Chemical Kinetics and Combustion

Language: English

Topic: melanin and skin pigmentation

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2083406213

FWCI: 0.839

Cites: 37

Cited by: 73

Related to: 10