Work

Semiautomated determination of minimum bactericidal concentrations of antibiotics

Year: 1983

Type: article

Abstract: Using a newly devised 50-channel photometer which records the opacity of growing bacterial cultures, it was shown that the time taken by cultures diluted 1/1000 in fresh broth to reach 50% of the opacity of a fully grown culture was inversely related to the concentration of organisms in the original culture. This relation was used to determine the numbers of survivors after exposure to benzylpenicillin and gentamicin alone and in combination. The procedure is commended as a labour-saving and potentially rapid method of obtaining comprehensive information on the bactericidal action and interaction of antibiotics.

Source: Journal of Clinical Pathology

DOI: 10.1136/jcp.36.2.208

Authors: F O'Grady, Barbara Murphy, NJ Pearson

Institutions: Nottingham University Hospitals NHS Trust, Queen's Medical Centre

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2083594852