Work

Directed Evolution of DNA Polymerases for Next‐Generation Sequencing

Year: 2010

Type: article

Abstract: Speeding evolution: An activity-based phage display method was used to address the title application. A mutant of Taq DNA polymerase was identified (see scheme) that incorporates the fluorophore-labeled dA, dT, dC, and dG substrates 50 to 400-fold more efficiently into scarred primers in solution and that also demonstrates significantly improved performance under actual sequencing conditions.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201001607

Authors: Aaron M. Leconte, Maha P. Patel, Lauryn E. Sass, Peter McInerney, Mirna Jarosz, Li Kung, Jayson L. Bowers, Philip R. Buzby, J. William Efcavitch, Floyd E. Romesberg

Institutions: Scripps Research Institute, Helicos Biosciences (United States)

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Human Genome Research Institute, National Institute of General Medical Sciences

Awards: High Accuracy Single Molecule DNA Sequencing by Synthesis, Expanding the Genetic Alphabet by Design and Selection, G284453737, G5448664165, Providing the $1000 genome via improved single molecule sequencing, G7702922093

OpenAlex ID: W2083616122

FWCI: 1.302

Cites: 28

Cited by: 31

Related to: 10