Work

Chromatin remodeling by nucleosome disassembly in vitro

Year: 2006

Type: article

Abstract: The RSC chromatin-remodeling complex completely disassembles a nucleosome in the presence of the histone chaperone Nap1 and ATP. Disassembly occurs in a stepwise manner, with the removal of H2A/H2B dimers, followed by the rest of the histones and the release of naked DNA. RSC and related chromatin-remodeling complexes may be responsible for the removal of promoter nucleosomes during transcriptional activation in vivo.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0511050103

Authors: Yahli Lorch, Barbara Maier-Davis, Roger D. Kornberg

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G2913654748, RNA Polymerase II Transcription System from Yeast, Gene Activation In Vitro