Description of the Sphingolipid Content and Subspecies in the Diabetic Cornea
Year: 2014
Type: article
Abstract: PURPOSE: Diabetes mellitus (DM) is characterized by high blood sugar levels over a prolonged period. Long term complications include but not limited heart disease, stroke, kidney failure, and ocular damage. An estimated 382 million people are diagnosed with Type 2 DM accounting for 90% of the cases. Common corneal dysfunctions associated with DM result in impaired vision due to decreased wound healing, corneal edema, and altered epithelial basement membrane. Lipids play a fundamental role in tissue metabolism and disease states. We attempt to determine the role of sphingolipids (SPL) in human Type I and Type II diabetic corneas. MATERIALS AND METHODS: Cadaver corneas from healthy (non-diabetic/no ocular trauma), Type I (T1DM), and Type II diabetic (T2DM) donors were obtained and processed for lipidomics using LC-MS/MS. RESULTS: Our data show significant differences in the SPL composition between control, T1DM and T2DM corneas. Both T1DM and T2DM showed a 10-folddownregulation of sphingomyelin(SM), 5-fold up regulation of Ceramides (Cer) and 2-fold upregulation of monohexosylceramides (MHC). Differences were also seen in total amounts of SPL where Cer was increased by approximately 3 fold in both T1DM and T2DM where SM decreased by 50% in both T1DM and T2DM when compared to healthy controls. No differences were seen in MHC amounts. CONCLUSIONS: Overall, our data indicate major differences in SPL distribution in human diabetic corneas. Information on the sphingolipids role in cornea, corneal cell physiology, and diseases are very limitedwhich highlights the importance of these findings.
Source: Current Eye Research
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1160139270, G1892013557, AB SCIEX 5500 QTRAP Mass Spectrometer for Lipidomics and Metabolomics Research, G3869733921, The functional role of the cPLA2alpha/C1P interaction in sepsis resolution, Transplantation of a self-assembled corneal substitute, G5856604648, Documentation of Novel Keratoconus Markers: In Vitro and In Vivo, Regulation of caspase 9 RNA splicing in NSCLC cancer, CONSTRUCTION OF SIGNALLING FACILITY, G8173979607, Massey Cancer Center - Cancer Center Support Grant
OpenAlex ID: W2083911683