Work

High efficiency polyoma DNA transfection of chloroquine treated cells

Year: 1983

Type: article

Abstract: Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate coprecipitation methods. Exposure to chloroquine increased the proportion of transfected mouse cells to approximately 40%. From a culture of one million such cells, microgram quantities of newly synthesized viral DNA could be isolated. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.

Source: Nucleic Acids Research

DOI: 10.1093/nar/11.5.1295

Authors: H. Luthman, G. Magnusson

Institution: Karolinska Institutet

Language: English

Topic: Polyomavirus and related diseases

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Cancerfonden, Svenska Sällskapet för Medicinsk Forskning, Knut och Alice Wallenbergs Stiftelse, Vetenskapsrådet