Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria
Year: 2000
Type: article
Abstract: The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
Authors: Stephan Heeb, Yoshifumi Itoh, Takayuki Nishijyo, Ursula Schnider, Christoph Keel, Julie Wade, Ultan Walsh, Fergal O'Gara, Dieter Haas
Institution: University of Lausanne
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2084879740