Work

Synthesis and NMR of RNA with Selective isotopic enrichment in the bases

Year: 1995

Type: article

Abstract: Efficient syntheses of pyrimidine and purine nucleosides and nucleotides with selective 13C enrichment in the base moieties are described. Uridine and cytidine are labeled at position C6 and adenosine and guanosine are labeled at position C8. The selectively labeled nucleosides were converted to nucleoside triphosphates and used with in vitro transcription to synthesize labeled RNA. Isotope-edited 12C and 13C sub-spectra of a omega 1-1/2-X-filtered NOESY experiment are demonstrated to be useful for making resonance assignments and for deriving structural information in large (> 20 nt) RNA molecules. The labeled RNAs also allow heteronuclear J-couplings and relaxation parameters to be measured without complications from 13C-13C J-couplings.

Source: Nucleic Acids Research

DOI: 10.1093/nar/23.23.4913

Authors: John SantaLucia, Ling X. Shen, Zhuoping Cai, Hal Lewis, Ignacio Tinoco

Institutions: Lawrence Berkeley National Laboratory, University of California, Berkeley

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze