Work

Serial studies of autologous antibody reactivity to melanoma. Relationship to clinical course and circulating immune complexes.

Year: 1985

Type: article

Abstract: The low titer and incidence of autologous antibody to melanoma has hampered its evaluation.Through acid dissociation and ultrafiltration of serum, we have been able to augment the autologous immune response in 9 of 10 patients studied.This result suggests that autologous antibody is present in most patients with melanoma, but is obscured by circulating antigen and the formation of immune complexes.Because native anti- body and antibody derived from circulating immune complexes are produced by the host against physiologically relevant antigens, correlations can be made to clinical course.Serological studies of three patients with melanoma were performed with serum samples obtained over many months; these studies demonstrated correlations with tumor progression and clinical course.Serial serologic studies may yet provide one of the better ways to evaluate these relationships.They have the advantage of detecting transient events that may occur with the inception of metastatic disease or autoimmune phenomena, and of avoiding the difficulties encountered in comparing antibody responses between different individuals. Methods Cell linesMelanoma cells.Tumor acquisition followed a protocol approved by the institutional review board.Preparation maintenance and identifi- cation of tumor lines have been reported previously (19).Melanoma cell line M 14 was kindly supplied by Dr. Darwin 0. Chee of Scott Laboratories, West Warwick, RI.Cultures established at three or more passages were used in serologic assays. Nonmelanoma cellsAcquisition and maintenance of nonmelanoma cell lines have been reported previously (19).Additional cell lines used for specificity testing include: breast carcinoma cell line (BCA), supplied by Dr.

Source: Journal of Clinical Investigation

DOI: 10.1172/jci112042

Authors: D R Vlock, J M Kirkwood

Institution: Yale University

Language: English

Topic: Cutaneous Melanoma Detection and Management

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Pfizer