Work

Two-Step Purification of Cordycepin from Cordyceps millitaris by High-Speed Countercurrent Chromatography

Year: 2009

Type: article

Abstract: Cordycepin is successfully isolated and purified from Cordyceps millitaris in two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatography analysis.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826070903188153

Authors: Xiuyun Ju, Yong Sun, Xiaoying Cao, Jihong Jiang, Tianyou Zhang, Yoichiro Ito

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009