Work

Simultaneous multiplane in vivo nonlinear microscopy using spectral encoding

Year: 2012

Type: article

Abstract: Conventional point-by-point imaging schemes for laser scanning microscopy limit acquisition speeds, particularly when imaging three-dimensional volumes. We report a novel approach that achieves parallelization of multiple fields of view through the use of spectral encoding. By focusing two or more beams of different wavelengths at different positions within a suitable tissue, fluorescence or second/third harmonic generation emissions from these regions can be uniquely separated. We demonstrate that this approach can allow simultaneous in vivo imaging of fluorescence in two planes within the living rodent cortex, and of second harmonic generation in fresh tissue.

Source: Optics Letters

DOI: 10.1364/ol.37.002967

Authors: Lauren E. Grosberg, Brenda R. Chen, Elizabeth M. C. Hillman

Institution: Columbia University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of Neurological Disorders and Stroke, National Science Foundation Graduate Research Fellowship Program

Awards: G5160976943, In-vivo optical imaging of neurovascular coupling and cerebral metabolism, CAREER: Interventional Microscopy for In-vivo Investigations of Brain Function, G9000240457