Work

Direct Microsensor Measurement of Nitric Oxide Production by the Osteoclast

Year: 1999

Type: article

Abstract: Nitric oxide (NO) triggers marked osteoclast retraction which closely resembles that due to Ca2+. The effect of Ca2+ has been attributed to a stimulated release of NO. Here, we show for the first time, by direct measurement with a microsensor, that osteoclasts do indeed produce NO and that this production is enhanced by a high Ca2+. We also show that the Ca2+ ionophore, A23187, mimics the latter. Furthermore, osteoclasts on dentine produce more NO than osteoclasts on glass and NO release from dentine-plated osteoclasts is much less sensitive to stimulation by Ca2+. Finally, the microsomal Ca2+ store-depleting agent, thapsigargin, attenuates NO release only from osteoclasts on glass, suggesting that stored Ca2+ has the dominant effect in modulating NO release from non-resorbing cells. NO is a powerful inhibitor of bone resorption: a direct demonstration of its production is therefore strong evidence for a role in modulating osteoclast function.

Source: Biochemical and Biophysical Research Communications

DOI: 10.1006/bbrc.1999.0703

Authors: Susan F. Silverton, Olugbenga A. Adebanjo, Baljit S. Moonga, Emmanuel M. Awumey, Tadeusz Malinski, Mone Zaidi

Institutions: University of Pennsylvania, Philadelphia VA Medical Center, Oakland University

Language: English

Topic: Bone Metabolism and Diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,110