Work

Rapid and Sensitive Detection of Lophophora williamsii by Loop-Mediated Isothermal Amplification

Year: 2009

Type: article

Abstract: We have developed a convenient method for the detection of Lophophora williamsii using loop-mediated isothermal amplification (LAMP). We designed six species-specific primers for L. williamsii, including two loop primers. This L. williamsii-specific primer set was used for LAMP of total DNA extracted from L. williamsii and from L. diffusa. Real-time monitoring of LAMP was achieved by measuring turbidity due to the formation of magnesium pyrophosphate. Amplification occurred in samples mixed with total DNA from L. williamsii, but not in those mixed with total DNA from L. diffusa. We could also visually observe L. williamsii by adding fluorescent detection reagent to the reaction tube and exposing it to UV light. This new method amplified L. williamsii selectively and is expected to be applicable to the detection of peyote.

Source: Biological and Pharmaceutical Bulletin

DOI: 10.1248/bpb.32.887

Authors: Yohei Sasaki, Tsuguto Fujimoto, Masako Aragane, Ichiro Yasuda, Seiji Nagumo

Institutions: Hoshi University, National Institute of Infectious Diseases, Tokyo Metropolitan Institute of Public Health, Chiba Institute of Science

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funder: Hoshi University

OpenAlex ID: W2088028614

FWCI: 0.194

Cites: 16

Cited by: 8

Related to: 10