Work

Geno/cytotoxicty and Apoptotic Properties of Phenolic Compounds from the Seeds of Dorema Glabrum Fisch. C.A

Year: 2015

Type: article

Abstract: INTRODUCTION: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anti-cancer potency of bioactive compounds from the seeds of Dorma glabrum. METHODS: Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds. RESULTS: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 μg/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments. CONCLUSION: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum.

Source: Bioimpacts

DOI: 10.15171/bi.2014.019

Authors: Morteza Eskandani, Elmira Dadizadeh, Hamed Hamishehkar, Hossein Nazemiyeh, Jaleh Barar

Institutions: Tabriz University of Medical Sciences, Research Center for Pharmaceutical Nanotechnology

Language: English

Topic: Plant chemical constituents analysis

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Tabriz University of Medical Sciences, University of Tabriz, Research Center for Pharmaceutical Nanotechnology