Work

Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

Year: 2000

Type: article

Abstract: To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed, validated, and used with a set of five 16S rRNA-targeted oligonucleotide probes. Application of the six probes to fecal samples from 27 human adults showed additivity of 70% of the total 16S rRNA detected by the bacterial domain probe. The Bacteroides group-specific probe accounted for 37% +/- 16% of the total rRNA, while the enteric group probe accounted for less than 1%. Clostridium leptum subgroup and Clostridium coccoides group-specific probes accounted for 16% +/- 7% and 14% +/- 6%, respectively, while Bifidobacterium and Lactobacillus groups made up less than 2%.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.66.5.2263-2266.2000

Authors: Abdelghani Sghir, Genevieve Gramet, Antonia Suau, Violaine Rochet, Philippe Pochart, Joel Dore

Institutions: Institut National de la Recherche Agronomique, Conservatoire National des Arts et Métiers

Language: English

Topic: Gut microbiota and health

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: European Commission