Work

Generating DNA Synbodies from Previously Discovered Peptides

Year: 2011

Type: article

Abstract: Synbody building: We demonstrate how off-the-shelf peptides can be transformed into high affinity protein capture reagents that mimic the recognition properties of natural antibodies (see figure). The designer synthetic antibody amplifies the binding affinity of the individual peptides by ∼1000-fold to bind Grb2 with a Kd of 2 nM, and functions with high selectivity in conventional pull-down assays from HeLa cell lysates.

Source: ChemBioChem

DOI: 10.1002/cbic.201100284

Authors: Rui Liu, Bing Jiang, Hanyang Yu, John C. Chaput

Institution: Arizona State University

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Awards: G1433160883, A High-throughput Route to Synthetic Antibodies for Array-based Cancer Detection