Year: 2002
Type: conference-abstract
Abstract: The crystal structure of a double mutant of interleukin 1-β (F42W/W120F), a cytokine secreted by macrophages, was determined by multi-wavelength anomalous dispersion (MAD) using data from highly twinned, selenomethionine-modified crystals.The space group is P43 with unit cell dimensions of a = b = 53.9Å and c = 77.4Å. Self-rotation function analysis, cumulative intensity distributions, analysis of second moments, and reduced |E 2 -1| values revealed the presence of merohedral twinning in crystals of both the native (twinning fraction α = 0.36) and SeMet (α = 0.4) forms.The structure was determined to 1.8 Å using the untreated, twinned intensity data (final overall figure of merit FOM = 0.53).The initial electron density maps were of sufficient quality for partial automated chain tracing.The structure was refined to 1.54 Å against twinned native data using SHELXL97.The results show that sufficient anomalous signal may still be extracted from twinned data in favorable cases for structure determination by MAD.
Institutions: Centre National de la Recherche Scientifique, Université Joseph Fourier, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Grenoble, Institut de Biologie Structurale, University of Göttingen, Scripps Research Institute, European Synchrotron Radiation Facility, University of California San Diego, European Molecular Biology Laboratory
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2088785231