Work

Products of RNA Polymerases in HeLa Cell Nuclei

Year: 1971

Type: article

Abstract: RNA polymerase activities in intact HeLa cell nuclei have been examined and compared to activities investigated in previous studies of the purified enzymes. The RNA synthesized by the mammalian polymerases while still in nuclei is identified. The polymerases are tentatively identified by location, sensitivity to alpha-amanitin, and response to manganese and altered ionic strength. Polymerase I is located in the nucleolus and labels partially complete precursor molecules of ribosomal RNA. Polymerases II and III are in the nucleoplasm and both label giant nuclear heterogeneous RNA.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.68.11.2861

Authors: Ester A. Zylber, Sheldon Penman

Institution: Massachusetts Institute of Technology

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, U.S. Public Health Service

OpenAlex ID: W2088877309

FWCI: 9.005

Cites: 24

Cited by: 225

Related to: 10