Work

Using Marine Natural Products to Discover a Protease that Catalyzes Peptide Macrocyclization of Diverse Substrates

Year: 2009

Type: article

Abstract: Small N-C terminally cyclic ribosomal peptides are common in nature, yet the mechanisms underlying their biosyntheses remain largely unknown. We recently identified candidate peptide cyclase genes in the metagenomes of marine animals. Here we report that PatG, a protease discovered in this analysis, cleaves variables, short peptides out of a precursor protein, and cyclizes these peptides in vivo and in vitro.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja8092168

Authors: Jaeheon Lee, John McIntosh, Brian J. Hathaway, Eric W. Schmidt

Institution: University of Utah

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Symbiont Models for Natural Product Pathway Manipulation, G635527312