Year: 2014
Type: article
Abstract: Among important components of American ginseng, protopanaxadiol (PPD) showed more active anticancer potential than other triterpenoid saponins. In this study, we determined the in vivo effects of PPD in a mouse cancer model first. Then, using human colorectal cancer cell lines, we observed significant cancer cell growth inhibition by promoting G1 cell cycle redistribution and apoptosis. Subsequently, we characterized the downstream genes targeted by PPD in HCT-116 cancer cells. Using Affymetrix high density GeneChips, we obtained the gene expression profile of the cells. Microarray data indicated that the expression levels of 76 genes were changed over two-fold after PPD, of which 52 were upregulated while the remaining 24 were downregulated. Ingenuity pathway analysis of top functions affected was carried out. Data suggested that by regulating the interactions between p53 and DR4/DR5, the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway played a key role in the action of PPD, a promising colon cancer inhibitory compound.
Institution: University of Chicago
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $2,070
Funders: U.S. Department of Defense, National Institutes of Health, U.S. Public Health Service, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Complementary and Alternative Medicine, National Center for Complementary and Integrative Health
Awards: G1111451179, G1557130165, G2568206356, G2744431007, G4119534169, G4168771144, G5290574874, G5681611326, G5850682332, G6400037178, G8302759699, SPECIFIC KILLING OF PROSTATE CANCERS WITH INACTIVATED RB PATHWAY, G8995673948, G9000047952, G9000146032, G9000177286, Center for Interdisciplinary Study of Inflammatory Intestinal DIsorders (C-IID), G95852423
OpenAlex ID: W2089163218