Work

Separation and Purification of Triterpene Saponins From Roots of Radix Phytolaccae by High-speed Countercurrent Chromatography Coupled With Evaporative Light Scattering Detection

Year: 2010

Type: article

Abstract: Coupled with evaporative light scattering detection, high-speed countercurrent chromatography was successfully applied for the first time to separation and purification of four triterpene saponins including esculentoside A, B, C and D from roots of Radix Phytolaccae. The separation was performed with an optimized two-phase solvent system composed of chloroform-methanol-water (4:4:2, v/v) using the lower phase as the mobile phase at a flow rate of 1.5 ml/min,. From 150 mg of crude extract 46.3 mg of esculentoside A, 21.8 mg of esculentoside B, 7.3 mg of esculentoside C, and 13.6 mg of esculentoside D were obtained at purities of 96.7%, 99.2%, 96.5% and 97.8%, respectively, as determined by HPLC analysis. The structures of the four triterpene saponins were identified by ESI-MS,(1)H NMR and (13)C NMR.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826070903574659

Authors: Jie Ma, Qianliang Chen, Daowan Lai, Wenji Sun, Tianyou Zhang, Yoichiro Ito

Institutions: Northwest University, Peking University, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009