Work

Intracellular Localization of the Neurotoxin 2,4-Diaminobutyric Acid in Lathyrus sylvestris L. Leaf Tissue

Year: 1987

Type: article

Abstract: The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA was vacuolar, based on the assumptions that each protoplast contained a single vacuole and that acid phosphatase occurred exclusively in the vacuole. DABA was not detectable in peroxisomal and mitochondrial fractions. Because the vacuole is not a major site of amino acid synthesis, this distribution implicates synthesis of DABA within chloroplasts with subsequent transport to and storage within the vacuoles of the mesophyll cells.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.83.4.900

Authors: Joyce G. Foster, W. Douglas Cress, Sara F. Wright, John L. Hess

Institutions: Agricultural Research Service, United States Department of Agriculture, Virginia Tech

Language: English

Topic: Plant Toxicity and Pharmacological Properties

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2089315686

FWCI: 0.689

Cites: 27

Cited by: 15

Related to: 10