Cloning and Differential Tissue-specific Expression of Three Mouse β Chemokine Receptor-like Genes, Including the Gene for a Functional Macrophage Inflammatory Protein-1α Receptor
Year: 1995
Type: article
Abstract: Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and RANTES, members of the beta chemokine family of leukocyte chemoattractants, bind to a common seven-transmembrane-domain human receptor. We have now cloned three related mouse genes: one for a selective MIP-1 alpha receptor (MIP-1 alpha R) and two for orphan receptors provisionally designated MIP-1 alpha receptor-like 1 and 2 (MIP-1 alpha RL1 and 2). Their deduced sequences are 80, 62, and 63% identical to the human MIP-1 alpha/RANTES receptor, respectively. K562 cells stably transfected with MIP-1 alpha R specifically bound 125I-human MIP-1 alpha and 125I-human RANTES with high affinity. The rank order of beta chemokine competition for 125I-human MIP-1 alpha binding was human MIP-1 alpha > mouse MIP-1 alpha approximately RANTES approximately MIP-1 beta > MCP-1. However, human RANTES was approximately 100-fold less potent as a calcium-mobilizing agonist for MIP-1 alpha R than either human or mouse MIP-1 alpha, which matched the selectively of mouse leukocytes for calcium mobilization by MIP-1 alpha and RANTES. No other beta or alpha chemokines tested were agonists for MIP-1 alpha R. RNA for all three genes was detected in mouse leukocytes, but unique patterns of expression were identified in solid organs: MIP-1 alpha R, heart, spleen, and lung; MIP-1 alpha RL1, skeletal muscle; and MIP-1 alpha RL2, spleen and liver. These data identify potentially important new targets for beta chemokine action in the mouse.
Source: Journal of Biological Chemistry
Authors: Ji-Liang Gao, Philip M. Murphy
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W2089629517