Work

Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids

Year: 2007

Type: article

Abstract: Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.

Source: Organic & Biomolecular Chemistry

DOI: 10.1039/b706507h

Authors: Hai Yu, Harshal A. Chokhawala, Ajit Varki, Xi Chen

Institutions: University of California, Davis, University of California San Diego

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Studies of naturally occurring structurally modified carbohydrates