Work

Blastocoel expansion in the preimplantation mouse embryo: Stimulation of sodium uptake by cAMP and possible involvement of cAMP-dependent protein kinase

Year: 1989

Type: article

Abstract: Elevating cAMP levels in mouse blastocysts increases the rate of blastocoel expansion (F. Manejwala, E. Kaji, and R. M. Schultz, 1986, Cell, 46, 95-103), which requires extracellular sodium (F. Manejwala, E. J. Crago, Jr., and R. M. Schultz, 1989, Dev. Biol. 133, 210-220). We report that cAMP analogs that can activate the cAMP-dependent protein kinase stimulate 22Na+ uptake by cavitating mouse blastocysts and that inhibitors of cAMP-dependent protein kinase activity inhibit the cAMP-stimulated increase in both the rate of blastocoel expansion and 22Na+ uptake.

Source: Developmental Biology

DOI: 10.1016/0012-1606(89)90282-0

Authors: Fazal M. Manejwala, Richard M. Schultz

Institution: University of Pennsylvania

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,550

Funder: Eunice Kennedy Shriver National Institute of Child Health and Human Development