Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
Year: 2011
Type: article
Abstract: We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures.
Source: Biochemistry
DOI: 10.1021/bi200549x
Authors: Alexei S. Soares, Matthew A. Engel, Richard Stearns, Sammy Datwani, Joe Olechno, Richard Ellson, John M. Skinner, Marc Allaire, Allen M. Orville
Institutions: Brookhaven National Laboratory, Stony Brook University
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2091220528