Work

Mitochondrial Membrane Potential Probes and the Proton Gradient: A Practical Usage Guide

Year: 2011

Type: review

Abstract: Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.

Source: BioTechniques

DOI: 10.2144/000113610

Authors: Seth W. Perry, John P. Norman, Justin Barbieri, Edward B. Brown, Harris A. Gelbard

Institutions: University of Rochester Medicine, University of Rochester

Language: English

Topic: Mitochondrial Function and Pathology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funders: National Institutes of Health, National Institute of Mental Health, National Institute on Drug Abuse, National Institute of Environmental Health Sciences