Work

Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

Year: 1996

Type: article

Abstract: We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

Source: Molecular Brain Research

DOI: 10.1016/0169-328x(95)00238-n

Authors: Stefanie Kaech, Jae Bum Kim, Michael Cariola, Evelyn Ralston

Institutions: National Institutes of Health, National Institute of Neurological Disorders and Stroke

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Science Foundation, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung