Year: 2014
Type: article
Abstract: Clostridium difficile toxin B (TcdB) is a key virulence factor of bacterium and induces intestinal inflammatory disease. Because of its potent cytotoxic and proinflammatory activities, we investigated the utility of TcdB in developing anti-tumor immunity. TcdB induced cell death in mouse colorectal cancer CT26 cells, and the intoxicated cells stimulated the activation of mouse bone marrow-derived dendritic cells and subsequent T cell activation in vitro. Immunization of BALB/c mice with toxin-treated CT26 cells elicited potent anti-tumor immunity that protected mice from a lethal challenge of the same tumor cells and rejected pre-injected tumors. The anti-tumor immunity generated was cell-mediated, long-term, and tumor-specific. Further experiments demonstrated that the intact cell bodies were important for the immunogenicity since lysing the toxin-treated tumor cells reduced their ability to induce antitumor immunity. Finally, we showed that TcdB is able to induce potent anti-tumor immunity in B16-F10 melanoma model. Taken together, these data demonstrate the utility of C. difficile toxin B for developing anti-tumor immunity.
Source: PLoS ONE
Authors: Tuxiong Huang, Shan Li, Guangchao Li, Yuan Tian, Haiying Wang, Lianfa Shi, Gregorio Perez-Cordon, Li Mao, Xiaoning Wang, Jufang Wang, Hanping Feng
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1860762379, Toxemia and systemic disease in Clostridium difficile infection, Epithelium, dendritic cells, and Clostridium difficile associated colitis, G7204811217, Development of Vaccines against Clostridium difficile Infection, Immunoprophylactic Strategies to Control Emerging Enteric Infections
OpenAlex ID: W2091942590