Work

Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation

Year: 2011

Type: article

Abstract: mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1101503108

Authors: Jie Fu, James B. Munro, Scott C. Blanchard, Joachim Frank

Institutions: Howard Hughes Medical Institute, Columbia University, Weill Cornell Medicine

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences

Awards: G3030999216, Structural Analysis of Macromolecular Assemblies, Imaging Protein Synthesis on the Ribosome using Single-Molecule FRET, Computational Technology for High-Throughput Cryo-EM, G9000014357