Work

Cdc42 and Par6–PKCζ regulate the spatially localized association of Dlg1 and APC to control cell polarization

Year: 2005

Type: article

Abstract: Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astrocytes. Activation of the Par6-PKCzeta complex by Cdc42 at the leading edge of migrating cells promotes both the localized association of APC with microtubule plus ends and the assembly of Dlg-containing puncta in the plasma membrane. Biochemical analysis and total internal reflection fluorescence microscopy reveal that the subsequent physical interaction between APC and Dlg1 is required for polarization of the microtubule cytoskeleton.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.200412172

Authors: Sandrine Etienne-Manneville, Jean-Baptiste Manneville, Sarah Nicholls, Michael A. Ferenczi, Alan Hall