Effects of Colchicine, Griseofulvin and Caffeine on Cell Shape and Septum Formation of Cultured Carrot Cells in Suspension
Year: 1979
Type: article
Abstract: Colchicine at the concentration of 10-4 M completely inhibited phragmoplast formation of culcured carrot cells and produced large spherical cells, whereas griseofulvin and caffeine produced large but irregular cells. In the presence of griseofulvin or a lower concentration of colchicine (10-5 M), septum formation was incomplete and highly irregular cross walls were formed. Caffeine also inhibited normal septum formation, but it did not induce intricate cross walls.Inhibition of cytokinesis by 10-4 M colchicine could be the result of the loss of cytoplasmic microtubules in the phragmoplast region. In cells treated with 10-5 M colchicine or 5 × 10-5-10-3 M griseofulvin, a considerable number of microtubules remained but the array of vesicles in the phragmoplast region was markedly disordered. Caffeine seemed to inhibit fusion of the vesicles aligned in the phragmoplast region.
Source: Cell Structure and Function
DOI: 10.1247/csf.4.11
Author: Shoji Okamura
Institution: University of Toyama
Language: English
Subfield: Cell Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2092747151