Work

The need for speed

Year: 2012

Type: review

Abstract: One of the key enabling features of coherent Raman scattering (CRS) techniques is the dramatically improved imaging speed over conventional vibrational imaging methods. It is this enhanced imaging acquisition rate that has guided the field of vibrational microscopy into the territory of real-time imaging of live tissues. In this feature article, we review several aspects of fast vibrational imaging and discuss new applications made possible by the improved CRS imaging capabilities. In addition, we reflect on the current limitations of CRS microscopy and look ahead at several new developments towards real-time, hyperspectral vibrational imaging of biological tissues. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).

Source: Journal of Biophotonics

DOI: 10.1002/jbio.201200002

Authors: Jeffrey L. Suhalim, John C. Boik, Bruce J. Tromberg, Eric O. Potma

Institution: University of California, Irvine

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources

Awards: G2326367487, A Laser Microbeam Biotechnology Resource, A Laser Microbeam Biotechnology Resource, Mathematical, Computational and Systems Biology, Cancer Center Support Grant