Work

Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss

Year: 2011

Type: article

Abstract: Nonlinear vibrational imaging of live cells and organisms is demonstrated by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produced a 12 times larger stimulated Raman loss signal than picosecond pulse excitation. The large signal allowed real-time imaging of the conversion of deuterated palmitic acid into lipid droplets inside live cells, and three-dimensional sectioning of fat storage in live C. elegans. With the majority of the excitation power contributed by the Stokes beam in the 1.0 to 1.2 μm wavelength range, photodamage of biological samples was not observed.

Source: The Journal of Physical Chemistry Letters

DOI: 10.1021/jz200516n

Authors: Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng

Institution: Purdue University West Lafayette

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering, Division of Chemical, Bioengineering, Environmental, and Transport Systems

Award: G64318696