Work

A Mitotic Septin Scaffold Required for Mammalian Chromosome Congression and Segregation

Year: 2005

Type: article

Abstract: Coordination of cytokinesis with chromosome congression and segregation is critical for proper cell division, but the mechanism is unknown. Here, septins, a conserved family of polymerizing guanosine triphosphate-binding proteins, localized to the metaphase plate during mitosis. Septin depletion resulted in chromosome loss from the metaphase plate, lack of chromosome segregation and spindle elongation, and incomplete cytokinesis upon delayed mitotic exit. These defects correlated with loss of the mitotic motor and the checkpoint regulator centromere-associated protein E (CENP-E) from the kinetochores of congressing chromosomes. Mammalian septins may thus form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation.

Source: Science

DOI: 10.1126/science.1106823

Authors: Elias T. Spiliotis, Makoto Kinoshita, W. James Nelson

Institutions: Kyoto University, Stanford Medicine, Stanford University

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G1309844508, Topogenesis of NA/K-ATPASE in Polarized Epithelial Cells, Topogenesis of Na/K-ATPase in Polarized MDCK Epithelial Cells