Work

Optical Methods for Single Molecule Detection and Analysis

Year: 2012

Type: other

Abstract: As analytical chemists, the highest resolution measurement one can make is at the single molecule level; it just does not get any better than that. To determine the concentration of a molecule in solution, the best way is to count the number of molecules in a given volume. As long as the volume contains a statistically large enough number of molecules and is above the Poisson noise limit, molecular counting is the most accurate way to make a measurement. Molecular counting is the method of the future and is beginning to be performed today.

Source: Analytical Chemistry

DOI: 10.1021/ac3027178

Author: David R. Walt

Institution: Tufts University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of Dental and Craniofacial Research, Army Research Office

Awards: Development of new tools for Single Cell Analysis, G1989475417, G4863771659, Microsensor Arrays for Saliva Diagnostics

OpenAlex ID: W2094632052

Cites: 22

Cited by: 224

Related to: 10