In Vivo Imaging of Tumors
Year: 2010
Type: preprint
Abstract: INTRODUCTION: Light microscopy of tumors, as for other thick, scattering tissues such as the brain or the developing embryo, is limited by light penetration and optical access. because of these problems, epifluorescence and confocal microscopy are typically limited to the outer 50-100 microm of the accessible tumor tissue. Most mouse tumors must be exteriorized for examination under the light microscope, a procedure that limits the duration and repeatability of imaging. this protocol describes the generation of chronic window preparations in the mouse. These preparations allow an implanted tumor to grow for several weeks in an optically accessible location in vivo, making it possible to examine the living tumor with high-resolution light microscopy in a repetitive manner. Two chronic window preparations are described: (1) the dorsal skinfold chamber, which allows in vivo imaging of tumors growing in the subcutaneous space, and (2) the cranial window, which allows in vivo imaging of tumors growing on the brain surface.
Source: Cold Spring Harbor Protocols
DOI: 10.1101/pdb.prot5452
Authors: Edward Brown, Lance L. Munn, Dai Fukumura, Rakesh K. Jain
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1169900800, G271430372, G2902221884, G3604496741, Nitric Oxide in Tumor Angiogenesis, Microcirculation and Radiation Therapy, G404075911, G446986368, Exploiting collagen organization to predict and prevent tumor metastasis, Doppler Optical Frequency Domain Imaging Instrument, Integrative Pathophysiology of Solid Tumors, Probing Tumor Microenvironment Using Nanotechnology, Flow-based remodeling and function of tumor vasculature
OpenAlex ID: W2094656362