Work

Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis

Year: 2002

Type: article

Abstract: A simple desalting procedure for the coupling of a polymer microchip injector to mass spectrometry is proposed. The overall process is based on the adsorption of proteins on a poly(vinylidene difluoride) (PVDF) membrane, which are then directly eluted in the spraying solution. This microchip-based approach has been successfully applied to small drugs, peptides and proteins originally diluted in phosphate-buffered saline (PBS). Moreover, when eluting the retained proteins in small volumes, a preconcentration is obtained. The combination of single-use, mass-produceable, low-sample-consumption, easy-to-automate, miniaturized polymer injectors with easy-to-handle solution-exchange membranes makes this system particularly amenable to screening applications.

Source: Electrophoresis

DOI: 10.1002/1522-2683(200210)23:20<3583::aid-elps3583>3.0.co;2-n

Authors: Niels Lion, Véronique Gobry, Henrik Jensen, Joël S. Rossier, Hubert Girault

Institutions: École Polytechnique Fédérale de Lausanne, DiagnoSwiss (Switzerland)

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2094874581

FWCI: 3.961

Cites:

Cited by: 47

Related to: 10