Work

PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers

Year: 2011

Type: article

Abstract: MOTIVATION: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task. RESULTS: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data. AVAILABILITY: PrimerProspector is open-source software available at http://pprospector.sourceforge.net CONTACT: [email protected] SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Source: Bioinformatics

DOI: 10.1093/bioinformatics/btr087

Authors: William A. Walters, J. Gregory Caporaso, Christian L. Lauber, Donna Berg-Lyons, Noah Fierer, Rob Knight

Institutions: Howard Hughes Medical Institute, Cooperative Institute for Research in Environmental Sciences, University of Colorado Boulder

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,989